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The 2nd response cycle in NO synthesis involves O2 websites binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as products (Fig. 9) - circular dichroism. As attracted Fig - https://www.figma.com/file/eT4jdyebIeUQ23ozOL89IX/Untitled?type=design&node-id=0%3A1&mode=design&t=vN0gsYYCmHohU5HF-1. 9, the reaction might also involve electron transfer from H4B to the Fe, IIO2 intermediate

l9 As displayed in Fig. 9, this might be because the H4B extreme serve as an electron sink in a later point in the reaction, so as to enable NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.